91制片厂
McNair Scholar, 2018
Major: Neuroscience and Behavior
Mentor: Dr. Aruturo Andrade, Department of Biological Sciences
Research Title: Dissecting Cell-Specific Roles Of 伪2未-Proteins In Seizure-Like Activity Of Drosophilia Melanogaster (fruit fly)
Abstract:
Lyrica and Gabapentin are anticonvulsant medications that interact with 伪2未-proteins to treat seizure-like activity. The 伪2未-protein subunits of voltage gated calcium channels are extracellular and dynamically aid in synaptic transmission. The need to understand the cell specific expression patterns of 伪2未-proteins may indicate a mechanistic function that links 伪2未- protein dysfunction to seizure-like behavior. The two conserved 伪2未-proteins of D. melanogaster (fruit fly), CG42817 and CG4587, will be used to investigate whether a2d protein disruption induces seizure-like behavior. Understanding the cell-specific role of 伪2未-proteins can provide insights on increasing the specificity of anticonvulsant drugs used to treat epilepsy. A bioinformatic analysis quantifying gene expression done by the Allen Institute for Brain Science漏 (2004) indicated a cell-specific expression of 伪2未 genes within inhibitory and excitatory cell populations. The relevance of this cell-specific expression is currently unknown but understanding the cell-specific role of 伪2未-proteins can provide insights for the creation of drugs that better target seizure-like symptoms. To assess the function of 伪2未-proteins in the fruit fly, we will knock down expression of the CG42817 and CG4587 伪2未 proteins within excitatory and inhibitory cell-specific populations. Transgenic fly lines expressing knocked down 伪2未-gene expression will be used to evaluate seizure-like behavior. Using the behavior elicited from flies with knocked down 伪2未 expression, we hope to answer whether either CG gene is sufficient to impair seizure-like activity in the fruit fly. If either CG gene is associated with seizure like behavior, this will reveal a2d proteins as effective targets for medications used to treat epilepsies.
